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Rezaie Kahkhaie, K., Rezaie Kehkhaie, A., Rezaie Kahkhaie, L., Koochakzai, M., Rezaie Keikhaie, K., Nakhaee Moghaddam, M. (2018). Isolation of Beta-Lactamase Producing Genes (SHV, CTX-M1, CTX-M2, CTX-M3) in Escherichia coli Isolated from Pregnant Woman Patients. Shahid Sadoughi University of Medical Sciences- Journal Management System, 1(1), 21-29. doi: 10.18502/wjpn.v1i1.2782
Kolsoum Rezaie Kahkhaie; Ashrafali Rezaie Kehkhaie; Leili Rezaie Kahkhaie; Maryam Koochakzai; Khadijeh Rezaie Keikhaie; Maryam Nakhaee Moghaddam. "Isolation of Beta-Lactamase Producing Genes (SHV, CTX-M1, CTX-M2, CTX-M3) in Escherichia coli Isolated from Pregnant Woman Patients". Shahid Sadoughi University of Medical Sciences- Journal Management System, 1, 1, 2018, 21-29. doi: 10.18502/wjpn.v1i1.2782
Rezaie Kahkhaie, K., Rezaie Kehkhaie, A., Rezaie Kahkhaie, L., Koochakzai, M., Rezaie Keikhaie, K., Nakhaee Moghaddam, M. (2018). 'Isolation of Beta-Lactamase Producing Genes (SHV, CTX-M1, CTX-M2, CTX-M3) in Escherichia coli Isolated from Pregnant Woman Patients', Shahid Sadoughi University of Medical Sciences- Journal Management System, 1(1), pp. 21-29. doi: 10.18502/wjpn.v1i1.2782
Rezaie Kahkhaie, K., Rezaie Kehkhaie, A., Rezaie Kahkhaie, L., Koochakzai, M., Rezaie Keikhaie, K., Nakhaee Moghaddam, M. Isolation of Beta-Lactamase Producing Genes (SHV, CTX-M1, CTX-M2, CTX-M3) in Escherichia coli Isolated from Pregnant Woman Patients. Shahid Sadoughi University of Medical Sciences- Journal Management System, 2018; 1(1): 21-29. doi: 10.18502/wjpn.v1i1.2782

Isolation of Beta-Lactamase Producing Genes (SHV, CTX-M1, CTX-M2, CTX-M3) in Escherichia coli Isolated from Pregnant Woman Patients

Journal of Integrated Maternal and Pediatric Care
Volume 1, Issue 1, July 2018, Pages 21-29    PDF (792.28 K)
DOI: 10.18502/wjpn.v1i1.2782
Authors
Kolsoum Rezaie Kahkhaie1; Ashrafali Rezaie Kehkhaie1; Leili Rezaie Kahkhaie1; Maryam Koochakzai2; Khadijeh Rezaie Keikhaie3; Maryam Nakhaee Moghaddam* 1
1Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran
2Department of Midwifery, Faculty of Nursing and Midwifery, Zabol University of Medical Sciences, Zabol, Iran
3Maternal and Fetal Health Research Center, Department of Obstetrics and Gynecology, Zabol University of Medical Sciences, Zabol, Iran
Abstract
Background: In recent decades, extended spectrum beta-lactamase (ESBL) generating bacteria have increased universally. Among the most important causative agents of nosocomial infections throughout the world, Escherichia coli as main ESBL-producing bacteria are so highly regarded. Trends in the treatment of infections by such bacteria have led to a global concern.
 
Methods: All strains were cultured and identified by the Clinical Microbiology Laboratory and were recovered from blood and urine cultures. In-vitro presence of ESBL was confirmed with Clinical and Laboratory Standard Institute double disc and PCR for CTX-M1, CTM-M2, CTX-M3 method.
 
Results: The results of this study showed that Escherichia coli samples were resistant to AN (42.85%), GM (28.57%), AM (35.71%), AMC (35.71%), CZ (35.71%), and AZM (50%) antibiotics. While the most susceptible to antibiotic was ampicillin (64.28%), the least resistance to antibiotics was gentamicin.
 
Conclusion: The current situation of multiple bacterial antibiotic resistance has become a worrisome issue in UTI. Multi-drug-resistant E. coli can be readily encountered in hospital settings during daily clinical practice, and urologist should act timely. The management of such infections is extremely important for the future, with particular reference to prevention of new antibiotic resistance patterns.
Keywords
E.coli; Extended-spectrum beta-lactamase; PCR; Gene CTX; SHV
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