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Rezaie Kahkhaie, K., Rezaie Kehkhaie, A., Rezaie Kahkhaie, L., Koochakzai, M., Rezaie Keikhaie, K., Nakhaee Moghaddam, M. (2018). Isolation of Beta-Lactamase Producing Genes (SHV, CTX-M1, CTX-M2, CTX-M3) in Escherichia coli Isolated from Pregnant Woman Patients. Shahid Sadoughi University of Medical Sciences- Journal Management System, 1(1), 21-29. doi: 10.18502/wjpn.v1i1.2782
Kolsoum Rezaie Kahkhaie; Ashrafali Rezaie Kehkhaie; Leili Rezaie Kahkhaie; Maryam Koochakzai; Khadijeh Rezaie Keikhaie; Maryam Nakhaee Moghaddam. "Isolation of Beta-Lactamase Producing Genes (SHV, CTX-M1, CTX-M2, CTX-M3) in Escherichia coli Isolated from Pregnant Woman Patients". Shahid Sadoughi University of Medical Sciences- Journal Management System, 1, 1, 2018, 21-29. doi: 10.18502/wjpn.v1i1.2782
Rezaie Kahkhaie, K., Rezaie Kehkhaie, A., Rezaie Kahkhaie, L., Koochakzai, M., Rezaie Keikhaie, K., Nakhaee Moghaddam, M. (2018). 'Isolation of Beta-Lactamase Producing Genes (SHV, CTX-M1, CTX-M2, CTX-M3) in Escherichia coli Isolated from Pregnant Woman Patients', Shahid Sadoughi University of Medical Sciences- Journal Management System, 1(1), pp. 21-29. doi: 10.18502/wjpn.v1i1.2782
Rezaie Kahkhaie, K., Rezaie Kehkhaie, A., Rezaie Kahkhaie, L., Koochakzai, M., Rezaie Keikhaie, K., Nakhaee Moghaddam, M. Isolation of Beta-Lactamase Producing Genes (SHV, CTX-M1, CTX-M2, CTX-M3) in Escherichia coli Isolated from Pregnant Woman Patients. Shahid Sadoughi University of Medical Sciences- Journal Management System, 2018; 1(1): 21-29. doi: 10.18502/wjpn.v1i1.2782

Isolation of Beta-Lactamase Producing Genes (SHV, CTX-M1, CTX-M2, CTX-M3) in Escherichia coli Isolated from Pregnant Woman Patients

World Journal of Peri and Neonatology
Volume 1, Issue 1, July 2018, Pages 21-29    PDF (792.28 K)
DOI: 10.18502/wjpn.v1i1.2782
Authors
Kolsoum Rezaie Kahkhaie1; Ashrafali Rezaie Kehkhaie1; Leili Rezaie Kahkhaie1; Maryam Koochakzai2; Khadijeh Rezaie Keikhaie3; Maryam Nakhaee Moghaddam* 1
1Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran
2Department of Midwifery, Faculty of Nursing and Midwifery, Zabol University of Medical Sciences, Zabol, Iran
3Maternal and Fetal Health Research Center, Department of Obstetrics and Gynecology, Zabol University of Medical Sciences, Zabol, Iran
Abstract
Background: In recent decades, extended spectrum beta-lactamase (ESBL) generating bacteria have increased universally. Among the most important causative agents of nosocomial infections throughout the world, Escherichia coli as main ESBL-producing bacteria are so highly regarded. Trends in the treatment of infections by such bacteria have led to a global concern.
 
Methods: All strains were cultured and identified by the Clinical Microbiology Laboratory and were recovered from blood and urine cultures. In-vitro presence of ESBL was confirmed with Clinical and Laboratory Standard Institute double disc and PCR for CTX-M1, CTM-M2, CTX-M3 method.
 
Results: The results of this study showed that Escherichia coli samples were resistant to AN (42.85%), GM (28.57%), AM (35.71%), AMC (35.71%), CZ (35.71%), and AZM (50%) antibiotics. While the most susceptible to antibiotic was ampicillin (64.28%), the least resistance to antibiotics was gentamicin.
 
Conclusion: The current situation of multiple bacterial antibiotic resistance has become a worrisome issue in UTI. Multi-drug-resistant E. coli can be readily encountered in hospital settings during daily clinical practice, and urologist should act timely. The management of such infections is extremely important for the future, with particular reference to prevention of new antibiotic resistance patterns.
Keywords
E.coli; Extended-spectrum beta-lactamase; PCR; Gene CTX; SHV
References
1. Gonzalez CM, Schaeffer AJ. Treatment of urinary tract infection: what’s old, what’s new, and what works. World J Urol 1999; 17(6): 372-82.
2. Foxman B, Barlow R, D'Arcy H, Gillespie B, Sobel JD. Urinary tract infection: estimated incidence and associated costs. Ann Epidemiol 2000; 10(8): 509-15.
3. Clermont O, Bonacorsi S, Bingen E. Rapid and simple determination of the Escherichiacoli phylogenetic group. Appl Environ Microbiol 2000; 66(10): 4555-8.
4. Toval F, Schiller R, Meisen I, Putze J, Kouzel IU, Zhang W, et al. Characterization of urinary tract infection associated Shiga toxin-producing Escherichia coli. Infect Immun 2014; 82(11): 4631-42.
5. Arbeloa A, Oates CV, Marches O, Hartland EL, Frankel G. Enteropathogenic and enterohemorrhagic Escherichia coli type III secretion effector EspV induces radical morphological changes in eukaryotic cells. Infect Immun 2011; 79(3): 1067-76.
6. Kong KF, Schneper L, Mathee K. Beta-lactam antibiotics: from antibiosis to resistance and bacteriology. APMIS 2010; 118(1): 1-36.
7. Lausova A, Bujdakova H, Kettner M. [Beta-Lactam antibiotics mechanisms of action and resistance in Enterobacteriaceae]. Epidemiol Mikrobiol Imunol 1997; 46(2): 73-80. [In Slovak].
8. Jacoby GA, Munoz-Price LS. The new beta-lactamases. N Engl J Med 2005; 352(4): 380-91.
9. Jacoby GA. AmpC beta-lactamases. Clin Microbiol Rev 2009; 22(1): 161-82.
10. Mohajeri P, Rostami Z, Farahani A, Norozi B. Distribution of ESBL producing Uropathogenic Escherichia coli and carriage of selected β-lactamase genes in Hospital and community isolates in west of Iran. Ann Trop Med Public Health 2014; 7(5): 219-22.
11. Mousavi S, Davari K, Amini P. Prevalence of (CTX-M-2) beta lactamase gene in E.coli isolated from patients with urinary tract infections (UTI) in Sanandaj, Iran. J Kurdistan Uni Med Sci 2012, 20(6): 107-15. [In Persian].
12. Mimira Scientific Co., Parviz M, Khalajzadeh S. Investigating the broad spectrum bla TEM-CTX-M bla SHV β-lactamase genes in Escherichia coli isolated from urinary tract infections and their antibiotic resistance. J Babol Univ of Med Sci 2015; 17(8): 26-19. [In Persian].
13. Mohammadi S, Ramezanzadeh R, Zandi S, Rohi S, Mohammadi B. Isolation and determination of antimicrobial resistance pattern of bacteria causing urinary tract infections in patients referred to Tohid Hospital in Sanandaj in 2012-2012. ZANKO 2015; 16(50): 62-55. [In Persian].
14. Sadeghi MR, Nahaei MR, Soltan Dallal MM. Resistance to Extended Spectrum β-Lactam antibiotics in Escherichia coli and Klebsiella pneumoniae from hospital. Tabriz Uni Med J 2008; 30(2): 79-86. [In Persian].
15. Shahcheraghi F, Nasiri S, Naviri H. Evalouation of presence the bla-SHV and bla-TEM β-Lactamase genes in clinical isolates resistant Escherichia coli to antibiotics from Tehran hospital. Iran J Med Microbiol 2007; 1(3): 1-8. [In Persian].
16. Mirzaee M, Pourmand MR, Chitsaz M, Mansouri S. Antibiotic resistance to third generation cephalosporins dueto CTX-M-Type extended-spectrum β-lactamases in clinical isolates of Escherichia coli. Iranian J Publ Health 2009; 38(1): 10-7.
17. Soltan Dallal MM, Sabbaghi A, Fallah Mehrabadi J, Molla Aghamirzaei H, Rastegar Lari A, Eshraghian MR, Fard Saneei A. Evaluation of presence the bla-SHV and bla-AmpC (CITM, FOX) β-lactamase genes in clinical isolates of Escherichia coli. J Med Counc I.R. Iran 2010; 28(3): 269-76. [In Persian].
18. Shayan S, Bokaeian M. Detection of ESBL- and AmpC-producing E. coli isolates from urinary tract infections. Adv Biomed Res 2015; 4: 220.
19. Saltan Dallalm MM, Azarsa M, Shirazi MH, Razgarglarari A, Owlia P, Fallah Mehranabadi J, et al. The prevalence of extended-spectrum beta-lactamases and CTX-M-1 producing Escherichia coli in urine samples collected at Tabriz city Hospitals. Tehran Univ Med J 2011; 69(5): 273-278. [In Persian].
20. Zaniani FR, Meshkat Z, Naderi Nasab M, Khaje-Karamadini M, Ghazvini K, Rezaee A, et al. The prevalence of TEM and SHV Genes among extended-spectrum beta-lactamases producing Escherichia coli and klebsiella pneumoniae. Iran J Basic Med Sci 2012; 15(1): 654-60.
21. Ghorbani-Dalini S, Kargar M, Doosti A, Abbasi P, Sarshar M. Molecular epidemiology of ESBL genes and multi-drug resistance in diarrheagenic Escherichia coli strains isolated from adults in Iran. Iran J Pharm Res 2015; 14(4): 1257-62.
22. Khaledi A, Esmaeili D, Esmaeili Farde Barzegar K, Ghamari N, Razipour H, Rostami H. Prevalence of Extended- Spectrum- β- Lactamase-producing Escherichia coli isolates among uropathogensin a Pediatrics Hospital. Der Pharma Chemica 2016, 8(3): 161-5.
23. Zamani K, Emami A, Bazargani A, Moattari A. Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran. Rev Soc Bras Med Trop 2015; 48(4): 479-82.
24. Farrokhnazar E, Moradi Bidhendi S, Karimi S. Prevalence of AmpC type extended spectrum beta lactamases genes in clinical Samples of E.coli Isolated from Poultry and Humans. Int J Med Res & Health Sci 2016; 7: 83-93.
25. Pakzad I, Ghafourian S, Taherikalani M, Sadeghifard N, Abtahi H, Rahbar M, et al. qnr prevalence in extended spectrum Beta-lactamases (ESBLs) and None-ESBLs Producing Escherichia coli isolated from urinary tract infections in central of Iran. Iran J Basic Med Sci 2011; 14(5): 458-64.
26. Sadegh Rezai M, Salehifar E, Rafiei A, Langaee T, Rafati M, Shafahi M, et al. Characterization of multidrug resistant extended-spectrum Beta-Lactamase-Producing Escherichia coli among uropathogens of pediatrics in North of Iran. Bio Med Res Int 2015; 309478.
27. Mohajeri P, Izadi P, Rezaei M, Fallahi B, Khademi H, Ebrahimi R. Investigating the production of broad-spectrum beta-lactamase in Escherichia coli isolated from Eredia infection and antimicrobial resistance pattern in Kermanshah. J Ardabil Uni Med Sci 2011; 11(1): 94-86. [In Persian].
28. Nakhaei Moghaddam M, Mahdi Forghanifard M, Moshrefi S. Prevalence and Molecular Characterization of Plasmid-mediated Extended-Spectrum β-Lactamase Genes (balaTEM, blaCTX and blASHV) Among Urinary Escherichia coli Clinical Isolates in Mashhad, Iran. Iran J Basic Med Sci 2012; 15(3): 833-9.
29. Mohammad Tabar M, Mirkalantari S, Izadi Amoli R. Detection of ctx-M gene in ESBL-producing E. coli strains isolated from urinary tract infection in Semnan, Iran. Electron Physician 2016; 8(7): 2686-90.
30. Nakhaei Moghaddam M, Zolfaghari M R, Tavakoli‐Hoseini N. CTX-M-15 Type β-lactamases From Clinical Isolates of Escherichia coli by Polymerase Chain Reaction and DNA Sequencing. Zahedan J Res Med Sci 2017; 19(3): e5814.
31. Nakhaei Moghaddam M, Hashemi Beidokhti M, Amel Jamehdar S, Ghahraman M. Genetic properties of blaCTX-M and blaPER β-lactamase genes in clinical isolates of Enterobacteriaceae by polymerase chain reaction. Iran J Basic Med Sci 2014; 17(5): 378-83.
32. Goudarzi M, Sabzehali F, Tayebi Z, Azad M, Boromani SH, Hashemi A, et al. Prevalence of bla CTX-M gene in multi resistant Escherichia coli isolated from urinary tract infections, Tehran, Iran. Novel Biomed 2014; 2(4): 107-13.
33. Altinkum SM, Ergin S, Bahar H and Torun MM. CTX-M-15 type extended spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae: A developing problem in infected outpatients and hospitalised patients in Istanbul, Turkey. Africa J Micro Res 2013; 7(8): 692-7.
34. Memariani M, Najar Peerayeh S, Zahraei Salehi T, Shokouhi Mostafavi SK. Occurrence of SHV, TEM and CTX-M β-Lactamase genes among enteropathogenic Escherichia coli strains isolated from children with diarrhea. Jundishapur J Microbiol 2015: 8(4): e15620.
35. Najar Peerayeh S, Rostami E, Eslami M, Ahangarzadeh Rezaee M. High Frequency of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae and Escherichia coli Isolates From Male Patients’ Urine. Arch Clin Infect Dis 2016; 11(2): e60127.
36. Nojoomi F, Ghasemian A, Eslami M, Khodaparast S. Antibiotic susceptibility profile, ESBL production and blaCTX-M1, blaSHV and blaTEM types among Escherichia coli blood isolates. Int J Enteric Pathog 2017; 5(1): 9-12.

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