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Human Embryonic and Mesenchymal Stem Cells: Report of a 1-day workshop held at Stem Cell Biology Research Center, Yazd, on 14th May 2025 | ||
| Regenerative Biomedicine | ||
| Volume 1, Issue 4, December 2025, Pages 344-347 PDF (809 K) | ||
| Document Type: Workshop Reports | ||
| DOI: 10.22034/jrb.2025.12.V1I4A8 | ||
| Author | ||
| Mahdieh Karimi* | ||
| Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran | ||
| Abstract | ||
| This report provides a brief summary of the proceedings from one-day workshop entitled “Human Embryonic and Mesenchymal Stem Cells” held at the Stem Cell Biology Research Center based in Yazd Reproductive Sciences Institute at Shahid Sadoughi University of Medical Sciences, Yazd, Iran on 14th May 2025. This workshop introduced participants to the biological characteristics, culture methods and laboratory handling of human embryonic stem cells (hESCs) and mesenchymal stem cells (MSCs). ESCs are derived from the inner cell mass during the blastocyst stage of the developing embryo in vitro. They are pluripotent and can theoretically give rise to any type of cells and also provide an ideal screening tool for potential drugs in the pharmaceutical industry and allow the study of early human development and infant cancers. Co-culture with a feeder layer of inactivated human or mouse embryonic fibroblasts is the conventional method for the hESCs culture and expansion that providing a facile stem cell expansion system with continuous medium conditioning while effectively preventing the interaction between hESCs and feeder cells. MSCs as a representative of adult stem cells can be isolated from almost all tissues and effectively expanded in vitro. These in vitro expanded cells have been shown to possess the potential to differentiate into specific cell lineages. | ||
| Keywords | ||
| Human Embryonic Stem Cells; Mesenchymal Stem Cells; Human Foreskin Fibroblast (HFFs); Co-culture; Workshop | ||
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