Statistics

Number of Journals8
Number of Issues52
Number of Articles383
Article View117,593
PDF Download85,900
Hasheminasab, H., Yavari, M., Kazemi, M. (2019). Assessment of the Frequency and Molecular Identity of Mycoplasma Species in Women with a History of Idiopathic Recurrent Abortion: A Case Control Study. Shahid Sadoughi University of Medical Sciences- Journal Management System, 2(2), 54-60. doi: 10.18502/wjpn.v2i2.4339
Hamid Hasheminasab; Mahdieh Yavari; Mohammad Javad Kazemi. "Assessment of the Frequency and Molecular Identity of Mycoplasma Species in Women with a History of Idiopathic Recurrent Abortion: A Case Control Study". Shahid Sadoughi University of Medical Sciences- Journal Management System, 2, 2, 2019, 54-60. doi: 10.18502/wjpn.v2i2.4339
Hasheminasab, H., Yavari, M., Kazemi, M. (2019). 'Assessment of the Frequency and Molecular Identity of Mycoplasma Species in Women with a History of Idiopathic Recurrent Abortion: A Case Control Study', Shahid Sadoughi University of Medical Sciences- Journal Management System, 2(2), pp. 54-60. doi: 10.18502/wjpn.v2i2.4339
Hasheminasab, H., Yavari, M., Kazemi, M. Assessment of the Frequency and Molecular Identity of Mycoplasma Species in Women with a History of Idiopathic Recurrent Abortion: A Case Control Study. Shahid Sadoughi University of Medical Sciences- Journal Management System, 2019; 2(2): 54-60. doi: 10.18502/wjpn.v2i2.4339

Assessment of the Frequency and Molecular Identity of Mycoplasma Species in Women with a History of Idiopathic Recurrent Abortion: A Case Control Study

Journal of Research in Maternal and Child Health
Volume 2, Issue 2, October 2019, Pages 54-60    PDF (673.31 K)
Document Type: Original Article
DOI: 10.18502/wjpn.v2i2.4339
Authors
Hamid Hasheminasab1; Mahdieh Yavari2; Mohammad Javad Kazemi* 3
1Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Yazd, Iran
2Department of Biology, Faculty of Science, Yazd University, Yazd, Iran
3Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Yazd, Iran
Abstract
Background: Abortion is the most common complication in the first trimester of pregnancy. Infections are reported as the etiology of abortions. Some species of Mycoplasmas are seen in the lower genital tract. The aim of this study was to investigate the frequency and the molecular identity of Mycoplasma species in women with a history of idiopathic recurrent abortion.
Methods: This study was carried out on 68 women with a history of recurrent abortion. Detecting 16S rRNA to identify the infection of Mycoplasma was done by polymerase chain reaction (PCR) on cervical samples. Furthermore, the sequencing of positive samples was performed to identify the species of Mycoplasma.
Results: The results showed that only one woman with history of three times abortions was infected by Mycoplasma. Her gestational age was six weeks and she had apparent signs of infection. After sequencing, Mycoplasma genitalium was diagnosed as the infectious agent. The prevalence rate of Mycoplasma was 1.47% and 0% in the case and control groups, respectively. There was no significant association betweenabortion and Mycoplasma infection.
Conclusion: It seems more studies are necessary to evaluate the relationship between Mycoplasma infection and abortion. Moreover the increase in the level of public health due to better education and the improvement of contraceptive methods compared to the past has created a significant reduction in Mycoplasma infection.
Keywords
Recurrent Abortion; Miscarriage; Mycoplasma; 16S rRNA
References
  1. Wilcox AJ, Weinberg CR, O'Connor JF, Baird DD, Schlatterer JP, Canfield RE, et al. Incidence of early loss of pregnancy. N Engl J Med 1988; 319(4): 189-94.
  2. Warburton D, Fraser FC. Spontaneous abortion risks in man: Data from reproductive histories collected in a medical genetics unit. Am J Hum Genet 1964; 16: 1-25.
  3. Regan L, Braude PR, Trembath PL. Influence of past reproductive performance on risk of spontaneous abortion. BMJ 1989; 299(6698): 541-5.
  4. Risch HA, Weiss NS, Clarke EA, Miller AB. Risk factors for spontaneous abortion and its recurrence. Am J Epidemiol 1988; 128(2):
    420-30.
  5. Daya S, Stephenson MD. Frequency of factors associated with habitual abortion in 197 couples. Fertil Steril 1996; 66(1): 24-9.
  6. Bornman MS, Mahomed MF, Boomker D, Schulenburg GW, Reif S, Crewe-Brown HH. Microbial flora in semen of infertile African men at Garankuwa hospital. Andrologia 1990; 22(2): 118-21.
  7. Gdoura R, Kchaou W, Chaari C, Znazen A, Keskes L, Rebai T, et al. Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis and Mycoplasma genitalium infections and semen quality of infertile men. BMC Infect Dis 2007; 7: 129.
  8. Heyborne KD, Witkin SS, McGregor JA. Tumor necrosis factor-alpha in midtrimester amniotic fluid is associated with impaired intrauterine fetal growth. Am J Obstet Gynecol 1992; 167(4 Pt 1): 920-5.
  9. Summers PR. Microbiology relevant to recurrent miscarriage. Clin Obstet Gynecol 1994; 37(3): 722-9.
  10. Boivin J, Bunting L, Collins JA, Nygren KG. International estimates of infertility prevalence and treatment-seeking: potential need and demand for infertility medical care. Hum Reprod 2007; 22(6): 1506-12.
  11. May MA, Kutish GF, Barbet AF, Michaels DL, Brown DR. Complete Genome Sequence of Mycoplasma synoviae Strain WVU 1853T. Genome Announc 2015; 3(3).
  12. Grad AI, Vica ML, Matei HV, Grad DL, Coman I, Tataru DA. Polymerase Chain Reaction as a Diagnostic Tool for Six Sexually Transmitted Infections - Preliminary Results. Clujul Med 2015; 88(1): 33-7.
  13. Verteramo R, Patella A, Calzolari E, Recine N, Marcone V, Osborn J, et al. An epidemiological survey of Mycoplasma hominis and Ureaplasma urealyticum in gynaecological outpatients, Rome, Italy. Epidemiol Infect 2013; 141(12): 2650-7.
  14. Hytten FE, Leitch I. The physiology of human pregnancy. 2nd ed. Hoboken, NJ: Blackwell Scientific Publications; 1964.
  15. Andrade-Rocha FT. Ureaplasma urealyticum and Mycoplasma hominis in men attending for routine semen analysis. Prevalence, incidence by age and clinical settings, influence on sperm characteristics, relationship with the leukocyte count and clinical value. Urol Int 2003; 71(4): 377-81.
  16. Parvege MM, Rahman M, Hossain MS. Genome-wide Analysis of Mycoplasma hominis for the Identification of Putative Therapeutic Targets. Drug Target Insights 2014; 8: 51-62.
  17. Najar-Peerayeh S, Sattari M. Detection of ureaplasma urealyticum and mycoplasma hominis in endocervical specimens from infertile women by polymerase chain reaction. Middle East Fertil Soc J 2006; 11(2): 104-8.
  18. Torfs CP, Christianson RE. Effect of maternal smoking and coffee consumption on the risk of having a recognized Down syndrome pregnancy. Am J Epidemiol 2000; 152(12): 1185-91.
  19. Shin JH, Joo HS, Lee WH, Seok HB, Calsamig M, Pijoan C, et al. Identification and characterization of cytopathogenic Mycoplasma hyorhinis from swine farms with a history of abortions. J Vet Med Sci 2003; 65(4): 501-9.
  20. Dehon PM, McGowin CL. Mycoplasma genitalium infection is associated with microscopic signs of cervical inflammation in liquid cytology specimens. J Clin Microbiol 2014; 52(7): 2398-405.
  21. Mohseni MN, Kheirkhah B, Mirshekari TR, Fasihi HM, Tafsiri E. Isolation and molecular identification of mycoplasma genitalium from the secretion of genital tract in infertile male and female. Iran J Reprod Med 2014; 12(9): 601-8.
  22. Labbe AC, Frost E, Deslandes S, Mendonca AP, Alves AC, Pepin J. Mycoplasma genitalium is not associated with adverse outcomes of pregnancy in Guinea-Bissau. Sex Transm Infect 2002; 78(4): 289-91.
  23. Jamalizadeh Bahaabadi S, Kheirkhah B, Farsinejad A, Habibzadeh V. Isolation and molecular identification of mycoplasma hominis from genital system of infertile men and women. J Microbial World 2014; 7(3):
    233-40.
  24. Wallach E, Friberg J. Mycoplasmas and Ureaplasmas in infertility and abortion. Fertility and sterility. 1980; 33(4): 351-9.
Statistics
Article View: 235
PDF Download: 173


Journal Management System. Powered by Sinaweb